Chromatograph or chromatography a term which defines a set of laboratory techniques used for the separation of mixtures as it involves passing of a mixture which is dissolved in a mobile phase through a stationery phase is very much utilized today. Chromatography is considered to be preparative or analytical as the purpose of preparative serves to separates the components of a mixture for its further use and that of analytical is served for measuring the relative proportions of analyses in a mixture. Another aspect which largely separates this term is known as thin layer chromatography which is simple, rapid, versatile and sensitive analytical technique for the separation of substance as later on which portrays its advantages and disadvantages.Few advantages of thin layer chromatography are that it is considered to be very inexpensive and also has multiple sample applications as the volume of solvent used per sample is very much less. This thin layer chromatography works on principles which are adsorption, partition, ion-exchange, size- exculsion etc these factors gain importance. Applications of high pressure thin layer chromatography includes in pharmaceuticals, forensics and in environment etc and the categories include drug monitoring in biological fluids and impurity profiles, poisons and adulterations, food products and pesticide residue in crops etc. The advancement and resolution is very much due to the use of high pressure thin layer chromatography plates as these are optimized with absorbent layers and smaller particle size distribution takes place in the stationery phase.
Thus the use of pressure in this equipment enables for faster analysis as the use of uv and florescent detectors in combination helps to analyze samples in sizes as small monogram and pictogram range and also the optimization of high pressure thin layer chromatography enables to separate the most complicated compounds by making the work easier. Apart from high pressure thin layer chromatography few other technique which are used for separating the molecules are high pressure liquid chromatography, ion exchange chromatography, affinity chromatography etc. Thus these chromatography techniques are used chemical techniques which are used for the separation of contaminants from chemical plants.
