A chromatographic technique which is used for separating a mixture of compounds which is used in biochemistry and analytical chemistry which helps to identify, quantify and purify the individual components of mixture is known as liquid chromatographs rather are called as high pressure liquid chromatographs. These high performance liquid chromatographs utilize different types of stationery phases which is a pump that moves with the help of the mobile phases and analyte through the column, also with high performance liquid chromatographs provides the high pressure which require to move the mobile phase and thus analyte through the densely packed column as the increased density arises from smaller particle sizes. In general term liquid chromatographs are used for separating ions or molecules that get dissolved in solvent.Columnar liquid chromatography is used for qualitative and quantitive analysis in such a manner which is very similar to the way in which the gas chromatography is employed as sometimes the retention volumes rather than retention times are used for qualitative analysis. Liquid chromatographic detectors are very much suitable for high performance columns which require clever technology. In this type of chromatography the separation of compounds are carried out on the basis of their idiosyncratic polarities and also interaction of these compounds which are in the stationery phase is also considered.
The equipments required for this liquid chromatographs are pumps, stationery phase and a detector which serves an important place for this type with which the smooth functioning of the equipment then takes place. Chromatography techniques has many advantages than that of analytical advantages as these are very much sensitive and reliable the main advantage is that the complex mixtures can be separated accurately by using few micrograms of samples and disadvantage of this chromatography is the machine is very much sensitive, has improper setup or contamination. Chromatography is considered to be extremely useful addition to a variety of fields which also includes pure and applied sciences, forensics and athletics which makes it unique and also interesting equipment.
